Summary
Fibroblasts in vitro can acquire myofibroblast phenotype by the development of several biochemical and morphological properties of smooth muscle cells, particularly the expresion of alpha-smooth muscle actin. These cells play a major role in inflammatory responses and in wound repair through their production of growth factors, cytokines, and other soluble mediators. Lipid bodies (LB) are lipid-rich cytoplasmic inclusions and have been recognized as specialized intracellular domains involved in the formation of paracrine mediators of inflammation. The aim of the present study was to investigate the occurrence and distribution of LB during differentiation of rat fetus skeletal fibroblasts into myofibroblasts in vitro. Primary cultures of fibroblasts were obtained from skeletal muscles of 18-d-old Wistar strain rat fetus by enzymatic dissociation. At 1–7 d, the cells were stained with Nile red vital dye to identify LB and then observed under a Zeiss CLSM-310. Our results showed that there was an accentuated increase in the number of LB during the differentiation of skeletal fibroblasts into myofibroblasts and that these inclusions were scattered at the cytoplasm.
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Arora, P. D.; McCulloch, C. A. G. The deletion of transforming growth factor β-induced myofibroblasts depends on growth conditions and actin organization. Am. J. Pathol. 155;2087–2099 1999.
Bandeira-Melo, C.; Bozza, P.T.; Weller, P.F. The cellular biology of eosinophil eicosanoid formation and function. J. Allergy Clin. Immunol. 109;393–400; 2002.
Boyle, J. E.; Lindross, P. M.; Rice, A. B.; Zhang, L.; Zeldin, D. C.; Bonner, J. C. Prostraglandin-E2 counteracts interleukin-1 beta-stimulated upregulation of platelet-derived growth factor alpha-receptor on rat pulmonary myofibrolbasts. Am. J. Respir. Cell Mol. Biol. 20(3):433–440; 1999.
Bozza, P. T.; Payne, J. L.; Morham, S. G.; Langenbach, R.; Smithies, O.; Weller, P. F. Leukocyte lipid body formation and eicosanoid generation: cyclooxygenase-independent inhibition by aspirin. Proc. Natl. Acad. Sci. USA 93:11091–11096; 1996.
Bozza, P. T.; Yu, W.; Cassara, J.; Weller, P. F. Pathways for eosinophill lipid body induction: differing signal transduction in cells form normal and hypereosinophilic subjects. J. Leukoc. Biol. 64:563–569; 1998.
Bozza, P. T.; Yu, W.; Penrose, J. F.; Morgan, E. L.; Dvorak, A. M.; Weller, P. F. Eosinophil lipid bodies: specific, inducible intracellular sites for enhanced eicosanoid formation. J. Exp. Med. 186(6):909–920; 1997.
Dugina, V.; Fotao, L.; Chaponier, C.; Vasiliev, J.; Gabbiani, G. Focal adhesion features during myofibroblastic differentiation are controlled by intracellular and extracellular factors. J. Cell Sci. 114:3285–3296; 2001.
Gailit, J.; Marchese, M. J.; Kew, R. R.; Gruber, B. L. The differentiation and function of myofibroblasts are regulated by mast cells mediators. J. Invest. Dermatol. 117:1113–1119; 2001.
Li, Y.; Huard, J. Differentiation of muscle-derived cells into myofibroblasts in injured skeletal muscle. Am. J. Pathol. 161:895–907; 2002.
Melo, R. C. N.; D'Ávila, H.; Fabrino, D. L.; Almeida, P. E.; Bozza, P. T. Macrophage lipid body induction by Chagas disease in vivo: putative intracellular domains for eicosanoid formation during infection. Tissue Cell 35:59–67;2003.
Pacheco, P.; Bozza, F. A.; Gomes, R. N.; Bozza, M.; Weller, P. F.; Castro-Faria-Neto, H. C.; Bozza, P. T. Lypopolysaccharide-induced leukocyte lipid body formation in vivo: innate immunity elicited intracellular loci involved in eicosanoid metabolism. J. Immunol. 169:6498–6506; 2002.
Powell, D. W.; Mifflin, R. C.; Valentich, J. D.; Crowe, S. E.; Saada, J. I.; West, A. B. Myofibroblasts. I. Paracrine cells important in health and disease. Am. J. Physiol. 277(46):1–19; 1999.
Seymour, L. S.; Zaidi, N. F.; Hollenberg, M. D.; Macnaughton, K. PAR1-dependent and independent increase in COX-2 and PGE-2 human colonic myofibrolbasts stimulated by trombin. Am. J. Physiol. Cell Physiol. 284:1185–1192; 2003.
Tomasek, J. T.; Gabbiani, G.; Hinz, B.; Chaponier, C.; Brown, R. A. Myofibroblasts and mechanoregulation of connective tissue remodeling. Nature 3:349–363; 2002.
Triggiani, M. A.; Oriente, A.; Seeds, M. C.; Bass, D. A.; Marone, G.; Chliton, F. H. Migration of human inflammatory cells into the lung results in the remodeling of arachidonic acid into a triglycerid pool. J. Exp. Med. 182:1181–1190; 1995.
Vaughan, M. B.; Howard, E. W.; Tomasek, J., Transforming growth factor-β1 promotes the morphological and functional differentiation of the myofibroblasts. Exp. Cell Res. 257:180–189; 2000.
Weller, P. F.; Bozza, P. T.; Yu, W.; Dvorak, A. M. Cytoplasmic lipid bodies in eosinophils: central role in eicosanoid generation. Int. Arch. Allergy Immunol. 118:450–452; 1999.
Weller, P. F.; Monahan-Earley, R. A.; Dvorak, H. F.; Dvorak, A. M. Cytoplasmic lipid bodies of human eosinophils: subcellular isolation and analysis of arachinodate incorporation. Am. J. Pathol. 138:141–148; 1991.
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Pereira, R.O., De Carvalho, T.M.U., Barbosa, H.S. et al. Enhancement of lipid bodies during differentiation of skeletal myofibroblasts of rat's fetus in vitro. In Vitro Cell.Dev.Biol.-Animal 40, 1–3 (2004). https://doi.org/10.1290/1543-706X(2004)40<1:EOLBDD>2.0.CO;2
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DOI: https://doi.org/10.1290/1543-706X(2004)40<1:EOLBDD>2.0.CO;2